FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-5to7kb-8_S31_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-5to7kb-8_S31_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341141
Sequences flagged as poor quality0
Sequence length35-151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN255047.476087600141877No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT5490.16093052432865002TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGGT350.009623709130.85034145
GATCGGA1450.059.0879971
GAAGAGC1600.053.377996
TCGGAAG1700.050.238113
CGGAAGA1850.049.715884
AGAGCAC1900.044.9498868
GGAAGAG2050.044.8655555
ATCGGAA2150.039.7421382
AAGAGCA2850.032.2717137
GAGCACA2950.031.1777579
GGGGGGG1800.002534956725.443121145
AGTTCCG1151.1734301E-814.95491130-34
CGTATGC1502.5616282E-711.81623345-49
TGCCGTC1751.375156E-711.02274750-54
CCGTCTT2101.657737E-510.904194145
CAGTTCC1752.4098154E-710.58347730-34
GTTCCGT1854.447811E-710.12172935-39
CGTCTGA1702.071707E-610.04762215-19
CCGTATC1752.3904468E-69.9358235-39
ACGTCTG1753.0008741E-69.76054715-19
Produced by FastQC (version 0.11.5)