FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-5to7kb-8_S31_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-5to7kb-8_S31_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341141
Sequences flagged as poor quality0
Sequence length35-151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4539413.306521350409362No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5730.16796573850695165Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGAGG1000.002036401871.51494145
CCCCAGT1300.005758148655.011497145
AGAGCGT1700.050.792598
GATCGGA1650.048.6020551
AAGAGCG1700.047.164557
GAGCGTC1800.044.5442929
GAAGAGC1900.042.199866
CGGAAGA1950.041.1178134
TCGGAAG1950.041.1178133
ATCGGAA2250.035.6354332
GGAAGAG2903.6379788E-1227.6481825
CGTGTCA1650.006614251518.6930981
GCCGTAT1651.2342207E-614.447463145
CTCGGTG1753.313653E-810.95028535-39
CGTCGTG1704.237263E-710.8859811
GCGTCGT1509.3544986E-710.68953910-14
AGCGTCG1551.4257803E-610.34471510-14
TCGGTGG1805.727961E-79.93636835-39
GTGGTCG2052.5124973E-79.53381140-44
TCTCGGT1951.7057937E-69.17203335-39
Produced by FastQC (version 0.11.5)