FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-8to10kb-4_S16_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-8to10kb-4_S16_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263614
Sequences flagged as poor quality0
Sequence length35-151
%GC36

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2646610.039679227962097No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGTGG1200.004249369359.389206145
ACCAAGG1300.005833343654.820805145
GGGGTGG2157.630806E-444.196617145
CCAAATT2700.005506349314.1526552
AGTGATT2800.007026172213.641439
Produced by FastQC (version 0.11.5)