FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-8to10kb-6_S24_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-8to10kb-6_S24_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99111
Sequences flagged as poor quality0
Sequence length35-151
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN67446.804491933286921No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGTT200.0021076268217.14743145
ACTAAGG300.007088858144.76495145
TCTACTT1150.00509435856.647156145
CATGTGC750.00235823135.14949
Produced by FastQC (version 0.11.5)