FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-8to10kb-7_S28_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-8to10kb-7_S28_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372443998
Sequences flagged as poor quality0
Sequence length35-151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4595203412.337971412281961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1846800.018.2281441
CGGAAGA1960450.017.2125114
TCGGAAG1966600.017.2064573
GAAGAGC2107150.016.0286546
AGATCGG1471600.014.483271
ATCGGAA2392450.014.4066152
AGAGCAC2463850.013.8369158
GGAAGAG2808150.012.2816515
GAGCACA3048550.011.3952219
AAGAGCA3051750.011.3872717
CGGGGGG210700.07.9185405145
GCGCCGG177250.07.5009046145
CCCGCGG163150.07.190435145
CGTATGC1779150.07.052011545-49
CGCGCGG130800.06.975735145
GGCGCGG164950.06.953926145
AATCTCG1722300.06.819388440-44
TATGCCG1825700.06.81929345-49
CCCCGGG382450.06.680071145
CGGCGCG114051.4551915E-116.6287613145
Produced by FastQC (version 0.11.5)