FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-8to10kb-7_S28_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-8to10kb-7_S28_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372443998
Sequences flagged as poor quality0
Sequence length35-151
%GC36

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4474152712.01295422674525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC2585000.013.0677329
GATCGGA2634350.012.872791
CGCGGGG375400.012.580061145
TCGGAAG2728900.012.5259633
AAGAGCG2772850.012.2424067
CGGAAGA2794500.012.2407784
AGAGCGT2773500.012.2395418
CGCGCGG298900.011.977253145
CGGGGGG662250.011.808448145
GAAGAGC2983050.011.4833676
ATCGGAA3100750.011.2584762
GGCGCGG400050.011.106772145
GCGCCGG406650.010.926507145
GCGGGGG837200.010.7055645145
GCGCGGG364200.010.596628145
CGGTGGG676100.010.36483145
CCCGCGG348950.010.332114145
GGAAGAG3742850.09.3389565
CCGCGGG315950.09.321883145
GCGTGGG506200.09.078634145
Produced by FastQC (version 0.11.5)