FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-8to10kb-8_S32_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-8to10kb-8_S32_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377720238
Sequences flagged as poor quality0
Sequence length35-151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4617974512.22591229014316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1823900.017.3649311
TCGGAAG1936500.016.4378493
CGGAAGA1943950.016.3606134
GAAGAGC2084600.015.306196
AGATCGG1468200.013.9979111
ATCGGAA2380300.013.6142042
AGAGCAC2437800.013.1359978
GGAAGAG2802350.011.634565
AAGAGCA3039750.010.8224397
GAGCACA3035800.010.7658839
CGCGCGG136200.07.439798145
CGGGGGG209800.07.306681145
CCCCGCG167350.06.986513145
CGTATGC1761400.06.779254445-49
GCGCCGG174650.06.694491145
CGCGCCG116453.6379788E-126.6935334145
AATCTCG1715150.06.549181540-44
TATGCCG1812350.06.52450145-49
CGCGGGG148550.06.2965627145
GAACTCC1776950.06.138084420-24
Produced by FastQC (version 0.11.5)