FastQCFastQC Report
Wed 28 Mar 2018
Geoduck-NMP-gDNA-8to10kb-8_S32_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-NMP-gDNA-8to10kb-8_S32_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377720238
Sequences flagged as poor quality0
Sequence length35-151
%GC36

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4471420011.83791481143777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC2490100.012.719599
GGCGCGG445150.012.655944145
GATCGGA2534300.012.6243721
TCGGAAG2650600.012.1645783
AAGAGCG2657350.012.0008127
CGGAAGA2687500.011.9629764
AGAGCGT2678250.011.8537138
GCGCGGG411550.011.672495145
CGCGCGG349700.011.651243145
GAAGAGC2882350.011.1887556
ATCGGAA3009200.010.9081732
CCGCGGG344600.010.801879145
GCGGGGG918750.010.731035145
GCGCCGG428950.010.495406145
CCCGCGG368850.010.2280855145
CCGCCGG436750.010.192794145
CGGGGGG743850.010.109711145
CGCGGGG410500.09.435403145
CCGGGGG537050.09.178976145
CGGTGGG716000.09.132996145
Produced by FastQC (version 0.11.5)