Bismark report for: /home/sam/analyses/20180503_oly_methylseq_trimgalore/zr1394_7_s456_trimmed.fq.gz (version: v0.19.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /home/sam/data/oly_methylseq/oly_genome/ with the specified options: -q --score-min L,0,-0.2 -p 16 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 1000000 Number of alignments with a unique best hit from the different alignments: 327795 Mapping efficiency: 32.8% Sequences with no alignments under any condition: 264497 Sequences did not map uniquely: 407708 Sequences which were discarded because genomic sequence could not be extracted: 671 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 74004 ((converted) top strand) CT/GA: 73152 ((converted) bottom strand) GA/CT: 90381 (complementary to (converted) top strand) GA/GA: 89587 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2002393 Total methylated C's in CpG context: 275174 Total methylated C's in CHG context: 8721 Total methylated C's in CHH context: 99298 Total methylated C's in Unknown context: 54 Total unmethylated C's in CpG context: 52432 Total unmethylated C's in CHG context: 404434 Total unmethylated C's in CHH context: 1162334 Total unmethylated C's in Unknown context: 405 C methylated in CpG context: 84.0% C methylated in CHG context: 2.1% C methylated in CHH context: 7.9% C methylated in Unknown context (CN or CHN): 11.8% Bismark completed in 0d 8h 1m 31s