FastQCFastQC Report
Wed 16 May 2018
EPI-151_S2_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-151_S2_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29567647
Sequences flagged as poor quality0
Sequence length101
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG2008810.6793946099261805TruSeq Adapter, Index 1 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT514100.173872476223759TruSeq Adapter, Index 1 (100% over 48bp)
CCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT391380.13236765171066875TruSeq Adapter, Index 1 (100% over 43bp)
CAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT349190.11809867724678937TruSeq Adapter, Index 1 (100% over 43bp)
CCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT297320.10055585417398957TruSeq Adapter, Index 1 (100% over 43bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTA296240.10019059007299431TruSeq Adapter, Index 1 (100% over 47bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)