FastQCFastQC Report
Wed 16 May 2018
EPI-153_S4_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-153_S4_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31826247
Sequences flagged as poor quality0
Sequence length101
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG2676400.8409411263602649TruSeq Adapter, Index 3 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTAT706120.22186719030993507TruSeq Adapter, Index 3 (100% over 48bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTA404810.1271937592893061TruSeq Adapter, Index 3 (100% over 47bp)
CCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT396230.12449787120674329TruSeq Adapter, Index 3 (100% over 43bp)
CCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT365600.11487373927563622TruSeq Adapter, Index 3 (100% over 43bp)
CAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT322370.10129061085964676TruSeq Adapter, Index 3 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)