FastQCFastQC Report
Wed 16 May 2018
EPI-181_S16_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-181_S16_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27579953
Sequences flagged as poor quality0
Sequence length101
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAATATTTGGGTTTTAATGGATATGTTAGTTGTTTTTTATATTTTTTG432200.1567080262972167No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG325800.11812928035084033TruSeq Adapter, Index 3 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)