FastQCFastQC Report
Wed 16 May 2018
EPI-188_S21_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-188_S21_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24209277
Sequences flagged as poor quality0
Sequence length101
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2795771.1548341571704102Illumina Single End PCR Primer 1 (100% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC506330.2091470967926882Illumina Single End PCR Primer 1 (100% over 49bp)
GGCGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG449520.18568088588519185Illumina Single End PCR Primer 1 (100% over 44bp)
GGGCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG300350.12406401066830702Illumina Single End PCR Primer 1 (100% over 44bp)
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG276820.11434459608190696Illumina Single End PCR Primer 1 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)