FastQCFastQC Report
Wed 16 May 2018
EPI-193_S22_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-193_S22_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32584046
Sequences flagged as poor quality0
Sequence length101
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG2406890.7386713117210797TruSeq Adapter, Index 9 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT610000.18720818157450428TruSeq Adapter, Index 9 (100% over 48bp)
CCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCT534130.1639237803678524TruSeq Adapter, Index 9 (100% over 43bp)
CCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCT516270.1584425703302776TruSeq Adapter, Index 9 (100% over 43bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTA370990.11385633324971368TruSeq Adapter, Index 9 (100% over 47bp)
CCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCT351330.1078227056271649TruSeq Adapter, Index 9 (100% over 43bp)
ACCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCT342410.10508516959496067TruSeq Adapter, Index 9 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)