FastQCFastQC Report
Wed 16 May 2018
EPI-199_S24_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-199_S24_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18024873
Sequences flagged as poor quality0
Sequence length101
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG1522420.8446217623835686TruSeq Adapter, Index 11 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAT403350.22377411480236226TruSeq Adapter, Index 11 (100% over 48bp)
CCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCT370700.20566025624702045TruSeq Adapter, Index 11 (100% over 43bp)
CCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCT363740.20179892529617266TruSeq Adapter, Index 11 (100% over 43bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTA235200.13048635627002753TruSeq Adapter, Index 11 (100% over 47bp)
CCGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCT198090.10989813908813671TruSeq Adapter, Index 11 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)