FastQCFastQC Report
Wed 16 May 2018
EPI-200_S25_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-200_S25_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27883056
Sequences flagged as poor quality0
Sequence length101
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1929130.6918646220127378TruSeq Adapter, Index 12 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT486670.17453969177553566TruSeq Adapter, Index 12 (100% over 48bp)
CCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT434790.1559334098816141TruSeq Adapter, Index 12 (100% over 43bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTA293240.10516781230866516TruSeq Adapter, Index 12 (100% over 47bp)
CCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT291890.10468364730178788TruSeq Adapter, Index 12 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)