FastQCFastQC Report
Wed 16 May 2018
EPI-206_S27_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-206_S27_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36712077
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4494831.2243464187547874Illumina Single End PCR Primer 1 (100% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC1000520.2725315704693036Illumina Single End PCR Primer 1 (100% over 49bp)
GGCTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG819730.22328619543917388Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG737220.20081130250407786Illumina Single End PCR Primer 1 (100% over 44bp)
GGGCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG685160.18663068286765688Illumina Single End PCR Primer 1 (100% over 44bp)
GGCCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG677690.18459593010768635Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG663110.18062448496171982Illumina Single End PCR Primer 1 (100% over 44bp)
GGCGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG597190.16266854092728122Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG569100.15501710785799452Illumina Single End PCR Primer 1 (100% over 45bp)
GGCGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG559010.15226869348743194Illumina Single End PCR Primer 1 (100% over 44bp)
GGGTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG557810.15194182557418365Illumina Single End PCR Primer 1 (100% over 44bp)
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG556740.1516503683515373Illumina Single End PCR Primer 1 (100% over 48bp)
GGGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG553350.1507269664966109Illumina Single End PCR Primer 1 (100% over 44bp)
GGCTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG546350.14882023700266264Illumina Single End PCR Primer 1 (100% over 44bp)
GGCGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG494690.1347485733373244Illumina Single End PCR Primer 1 (100% over 45bp)
GGGGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG491610.13390961235998716Illumina Single End PCR Primer 1 (100% over 44bp)
GGCAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG466620.12710258806659183Illumina Single End PCR Primer 1 (100% over 44bp)
GGCCGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG461410.12568343654323888Illumina Single End PCR Primer 1 (100% over 44bp)
GGCGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG428170.11662919534626166Illumina Single End PCR Primer 1 (100% over 44bp)
GTGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG413420.11261144391258496Illumina Single End PCR Primer 1 (100% over 44bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC413240.11256241372559772Illumina Single End PCR Primer 1 (100% over 49bp)
GGGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG412720.11242077096319013Illumina Single End PCR Primer 1 (100% over 45bp)
GCGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT410340.11177248293524772Illumina Single End PCR Primer 1 (100% over 46bp)
GGCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG400370.10905675535600996Illumina Single End PCR Primer 1 (100% over 44bp)
GGGCCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG400080.10897776227697495Illumina Single End PCR Primer 1 (100% over 44bp)
GGTGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG397770.10834854154397203Illumina Single End PCR Primer 1 (100% over 44bp)
GCGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG393460.10717454095555531Illumina Single End PCR Primer 1 (100% over 44bp)
GGCCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG390250.10630016928761617Illumina Single End PCR Primer 1 (100% over 44bp)
TGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG386630.10531411774931722Illumina Single End PCR Primer 1 (100% over 48bp)
GGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG376280.10249488199755084Illumina Single End PCR Primer 1 (100% over 44bp)
GCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG375010.10214894678936308Illumina Single End PCR Primer 1 (100% over 48bp)
GGCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG374270.10194737824285997Illumina Single End PCR Primer 1 (100% over 45bp)
GGGCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG372220.10138897889106083Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG369030.10052005502167584Illumina Single End PCR Primer 1 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)