FastQCFastQC Report
Wed 16 May 2018
EPI-208_S28_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-208_S28_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39075361
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG5223271.336717017150526TruSeq Adapter, Index 3 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTAT1207640.30905408653806166TruSeq Adapter, Index 3 (100% over 48bp)
CCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT852050.21805300787880116TruSeq Adapter, Index 3 (100% over 43bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTA720090.1842823665787758TruSeq Adapter, Index 3 (100% over 47bp)
CCGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT638120.16330495321591526TruSeq Adapter, Index 3 (100% over 43bp)
ACCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT544330.13930261578389513TruSeq Adapter, Index 3 (100% over 43bp)
ACCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT524110.13412799948284548TruSeq Adapter, Index 3 (100% over 43bp)
CCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT520360.1331683154507517TruSeq Adapter, Index 3 (100% over 43bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTAT495350.1267678627460409TruSeq Adapter, Index 3 (100% over 48bp)
ACCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT477930.12230981052228794TruSeq Adapter, Index 3 (100% over 43bp)
CCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC466290.11933095128666886TruSeq Adapter, Index 3 (100% over 44bp)
CCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCG429360.10987998293860932TruSeq Adapter, Index 3 (100% over 45bp)
AGCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT428590.10968292781735273TruSeq Adapter, Index 3 (100% over 43bp)
CCTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT397440.10171115245742708TruSeq Adapter, Index 3 (100% over 43bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTAT396500.10147059166004889TruSeq Adapter, Index 3 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)