FastQCFastQC Report
Wed 16 May 2018
EPI-215_S31_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-215_S31_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24211042
Sequences flagged as poor quality0
Sequence length101
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG1784790.7371801676276469TruSeq Adapter, Index 6 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTAT395300.16327260924994472TruSeq Adapter, Index 6 (100% over 48bp)
CCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT365390.15091874195253555TruSeq Adapter, Index 6 (100% over 43bp)
CCGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT265670.10973092360089251TruSeq Adapter, Index 6 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)