FastQCFastQC Report
Wed 16 May 2018
EPI-221_S33_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-221_S33_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24207549
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG3921641.6200070482145879TruSeq Adapter, Index 8 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT968280.39999092844963363TruSeq Adapter, Index 8 (100% over 48bp)
CCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT688920.2845889106741042TruSeq Adapter, Index 8 (100% over 43bp)
CCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT587900.24285812661166153TruSeq Adapter, Index 8 (100% over 43bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTA561230.2318409021912958TruSeq Adapter, Index 8 (100% over 47bp)
CCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT493650.20392399081790563TruSeq Adapter, Index 8 (100% over 43bp)
ACCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT480340.1984257059646972TruSeq Adapter, Index 8 (100% over 43bp)
CCGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT430280.17774620635901636TruSeq Adapter, Index 8 (100% over 43bp)
AGCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT395350.16331682319428537TruSeq Adapter, Index 8 (100% over 43bp)
ACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC393340.1624865036935379TruSeq Adapter, Index 8 (100% over 44bp)
CAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT374310.15462531956457054TruSeq Adapter, Index 8 (100% over 43bp)
CAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT368820.1523574319729767TruSeq Adapter, Index 8 (100% over 43bp)
CACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT358050.14790840658837456TruSeq Adapter, Index 8 (100% over 43bp)
CCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT343090.14172851617485108TruSeq Adapter, Index 8 (100% over 43bp)
CCACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT330490.13652352825971767TruSeq Adapter, Index 8 (100% over 43bp)
CCTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT326300.1347926632307963TruSeq Adapter, Index 8 (100% over 43bp)
ACCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT323910.13380536790403688TruSeq Adapter, Index 8 (100% over 43bp)
ACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG320570.13242563301224752TruSeq Adapter, Index 8 (100% over 45bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT308280.12734870432359757TruSeq Adapter, Index 8 (100% over 48bp)
ACCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT306720.12670427724839056TruSeq Adapter, Index 8 (100% over 43bp)
ACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC296720.12257333445860215TruSeq Adapter, Index 8 (100% over 44bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTA296460.12246592994606764TruSeq Adapter, Index 8 (100% over 47bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTA287960.11895462857474748TruSeq Adapter, Index 8 (100% over 47bp)
CCCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT273340.11291519021607681TruSeq Adapter, Index 8 (100% over 43bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG270110.11158089569497515TruSeq Adapter, Index 8 (100% over 45bp)
CACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT264020.109065151535994TruSeq Adapter, Index 8 (100% over 43bp)
ACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG259390.10715252502432196TruSeq Adapter, Index 8 (100% over 45bp)
GCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG257850.10651635983469454TruSeq Adapter, Index 8 (100% over 45bp)
CCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC253020.10452111446722673TruSeq Adapter, Index 8 (100% over 44bp)
CCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC250880.10363709271021201TruSeq Adapter, Index 8 (100% over 44bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT248480.10264566644066278TruSeq Adapter, Index 8 (100% over 48bp)
AGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC244820.10113374137960023TruSeq Adapter, Index 8 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)