FastQCFastQC Report
Wed 16 May 2018
EPI-221_S33_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-221_S33_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24207549
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3644951.505707992163932Illumina Single End PCR Primer 1 (100% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC855610.3534475960370874Illumina Single End PCR Primer 1 (100% over 49bp)
GGCGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG646510.26706958230261146Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG546510.22576015440472721Illumina Single End PCR Primer 1 (100% over 44bp)
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG491290.20294908831951555Illumina Single End PCR Primer 1 (100% over 48bp)
GGCTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG469260.19384862135361164Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG461100.19047777203714428Illumina Single End PCR Primer 1 (100% over 44bp)
GGGCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG408810.1688770721893406Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG389910.16106959031664048Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG372320.15380326194940264Illumina Single End PCR Primer 1 (100% over 45bp)
GGCCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG359620.14855696460637133Illumina Single End PCR Primer 1 (100% over 44bp)
GGGCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG353260.1459296849920659Illumina Single End PCR Primer 1 (100% over 44bp)
GGCGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG338620.13988198474781566Illumina Single End PCR Primer 1 (100% over 44bp)
GGCCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG327340.1352222812809343Illumina Single End PCR Primer 1 (100% over 44bp)
GGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG314930.1300957812788069Illumina Single End PCR Primer 1 (100% over 44bp)
GGGTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG311890.12883997467071118Illumina Single End PCR Primer 1 (100% over 44bp)
GGCAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG307880.12718346661200602Illumina Single End PCR Primer 1 (100% over 44bp)
GCGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT304430.125758291349529Illumina Single End PCR Primer 1 (100% over 46bp)
GCGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG294860.1218049790997015Illumina Single End PCR Primer 1 (100% over 44bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC292760.12093748111384595Illumina Single End PCR Primer 1 (100% over 49bp)
GGCGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG282530.11671152663989239Illumina Single End PCR Primer 1 (100% over 45bp)
GCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG268630.11096951616208646Illumina Single End PCR Primer 1 (100% over 48bp)
TGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG268620.11096538521929668Illumina Single End PCR Primer 1 (100% over 48bp)
GTGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG255890.10570669504789601Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG249620.10311659391869868Illumina Single End PCR Primer 1 (100% over 44bp)
GGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT245980.10161293074321567Illumina Single End PCR Primer 1 (100% over 46bp)
GGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT243220.10047279053323407Illumina Single End PCR Primer 1 (100% over 46bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)