FastQCFastQC Report
Wed 16 May 2018
EPI-44_S41_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-44_S41_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38920722
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG11640002.9906947769365635TruSeq Adapter, Index 8 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT2919670.7501582319053588TruSeq Adapter, Index 8 (100% over 48bp)
CCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT1943680.4993946412402113TruSeq Adapter, Index 8 (100% over 43bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTA1614250.4147533542671691TruSeq Adapter, Index 8 (100% over 47bp)
CAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT1542620.3963492763572063TruSeq Adapter, Index 8 (100% over 43bp)
CCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT1480480.38038348826108626TruSeq Adapter, Index 8 (100% over 43bp)
CCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT1455220.3738933722760847TruSeq Adapter, Index 8 (100% over 43bp)
ACCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT1328960.3414530696527161TruSeq Adapter, Index 8 (100% over 43bp)
CAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT1298410.3336037805259625TruSeq Adapter, Index 8 (100% over 43bp)
CACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT1278500.3284882536351715TruSeq Adapter, Index 8 (100% over 43bp)
ACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC1268670.32596260675739774TruSeq Adapter, Index 8 (100% over 44bp)
CCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT1173770.30157970861897165TruSeq Adapter, Index 8 (100% over 43bp)
ACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC1130990.2905881345161069TruSeq Adapter, Index 8 (100% over 44bp)
AGCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT1122460.2883964999415992TruSeq Adapter, Index 8 (100% over 43bp)
ACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG1109520.28507179286139656TruSeq Adapter, Index 8 (100% over 45bp)
CCACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT1096560.2817419471303744TruSeq Adapter, Index 8 (100% over 43bp)
ACGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT1037560.26658292721291243TruSeq Adapter, Index 8 (100% over 43bp)
ACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG1001110.2572177360944126TruSeq Adapter, Index 8 (100% over 45bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTA992170.2549207591781057TruSeq Adapter, Index 8 (100% over 47bp)
CCCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT935370.2403269908507864TruSeq Adapter, Index 8 (100% over 43bp)
CAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC874960.22480569605055115TruSeq Adapter, Index 8 (100% over 44bp)
CAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG872750.22423787513499877TruSeq Adapter, Index 8 (100% over 45bp)
CCTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT864640.2221541522277002TruSeq Adapter, Index 8 (100% over 43bp)
CACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT846300.2174420094262383TruSeq Adapter, Index 8 (100% over 43bp)
CCGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT842290.21641170993693284TruSeq Adapter, Index 8 (100% over 43bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT829150.21303561634853535TruSeq Adapter, Index 8 (100% over 48bp)
ACAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT826290.2123007892813499TruSeq Adapter, Index 8 (100% over 43bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTA825670.21214149110594607TruSeq Adapter, Index 8 (100% over 47bp)
AGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC800570.2056924843275004TruSeq Adapter, Index 8 (100% over 44bp)
CCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC798870.20525569900784474TruSeq Adapter, Index 8 (100% over 44bp)
CCCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT749450.19255809283291303TruSeq Adapter, Index 8 (100% over 43bp)
CCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG723240.18582389093398627TruSeq Adapter, Index 8 (100% over 45bp)
CGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGT715930.18394571405946683TruSeq Adapter, Index 8 (100% over 46bp)
CCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC711520.1828126415537718TruSeq Adapter, Index 8 (100% over 44bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTA710920.18265848202918744TruSeq Adapter, Index 8 (100% over 47bp)
ACCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT708390.18200844270052338TruSeq Adapter, Index 8 (100% over 43bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT700790.18005575538912152TruSeq Adapter, Index 8 (100% over 48bp)
ACCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT683920.17572130342289127TruSeq Adapter, Index 8 (100% over 43bp)
GCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG682970.17547721750896603TruSeq Adapter, Index 8 (100% over 45bp)
CGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG677660.17411290571639448TruSeq Adapter, Index 8 (100% over 45bp)
AGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC676020.17369153634919723TruSeq Adapter, Index 8 (100% over 44bp)
AGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT674010.17317510194183963TruSeq Adapter, Index 8 (100% over 43bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG656170.1685914254108647TruSeq Adapter, Index 8 (100% over 45bp)
CCAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT640230.16449592070774022TruSeq Adapter, Index 8 (100% over 43bp)
AACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT630590.1620190910127515TruSeq Adapter, Index 8 (100% over 43bp)
CCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC612000.15724271507604612TruSeq Adapter, Index 8 (100% over 44bp)
CAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC609690.15664920090639634TruSeq Adapter, Index 8 (100% over 44bp)
ACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG591210.1519010875491981TruSeq Adapter, Index 8 (100% over 45bp)
CCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT587060.15083481750415628TruSeq Adapter, Index 8 (100% over 43bp)
CACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGT577380.14834771050752862TruSeq Adapter, Index 8 (100% over 46bp)
ACCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT563470.14477377886258122TruSeq Adapter, Index 8 (100% over 43bp)
CACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC550760.14150816626680257TruSeq Adapter, Index 8 (100% over 44bp)
CGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC529030.135925022151439TruSeq Adapter, Index 8 (100% over 44bp)
GCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT526580.13529553742605288TruSeq Adapter, Index 8 (100% over 43bp)
CCACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATC519790.1335509654728399TruSeq Adapter, Index 8 (100% over 42bp)
ACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTA517990.13308848689908684TruSeq Adapter, Index 8 (100% over 47bp)
CCACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT507420.13037270994099237TruSeq Adapter, Index 8 (100% over 43bp)
CACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC505270.1298203049778984TruSeq Adapter, Index 8 (100% over 44bp)
TGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC499050.12822218457304055TruSeq Adapter, Index 8 (100% over 44bp)
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTA496580.12758756119683493TruSeq Adapter, Index 8 (100% over 47bp)
CTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT491700.12633373039688214TruSeq Adapter, Index 8 (100% over 43bp)
CCCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT484600.12450950935596725TruSeq Adapter, Index 8 (100% over 43bp)
ATGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT484400.12445812284777247TruSeq Adapter, Index 8 (100% over 43bp)
CCCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT484100.12438104308548027TruSeq Adapter, Index 8 (100% over 43bp)
CCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC476530.12243606375030762TruSeq Adapter, Index 8 (100% over 44bp)
AGCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT474710.12196844652573506TruSeq Adapter, Index 8 (100% over 43bp)
CCCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT468400.12034720219218954TruSeq Adapter, Index 8 (100% over 43bp)
ACTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT467460.12010568560367405TruSeq Adapter, Index 8 (100% over 43bp)
AGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG464660.11938627448894704TruSeq Adapter, Index 8 (100% over 45bp)
CCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG461840.11866172472340057TruSeq Adapter, Index 8 (100% over 45bp)
ACCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT453450.11650606070462927TruSeq Adapter, Index 8 (100% over 43bp)
CCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT451640.11604101280546644TruSeq Adapter, Index 8 (100% over 43bp)
GCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGT451600.1160307355038275TruSeq Adapter, Index 8 (100% over 46bp)
AACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT450540.11575838701039513TruSeq Adapter, Index 8 (100% over 43bp)
AGCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT444150.11411658807357172TruSeq Adapter, Index 8 (100% over 43bp)
CCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGT443230.11388021013587568TruSeq Adapter, Index 8 (100% over 46bp)
TGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC442290.11363869354736018TruSeq Adapter, Index 8 (100% over 44bp)
CGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC436980.11227438175478863TruSeq Adapter, Index 8 (100% over 44bp)
TGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT433910.11148559885399865TruSeq Adapter, Index 8 (100% over 43bp)
CCAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT427860.10993115698110638TruSeq Adapter, Index 8 (100% over 43bp)
AAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC412470.10597696517551755TruSeq Adapter, Index 8 (100% over 44bp)
CCAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATC411080.1056198289435638TruSeq Adapter, Index 8 (100% over 42bp)
AAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT399260.10258288630925193TruSeq Adapter, Index 8 (100% over 43bp)
ACCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT397050.10201506539369953TruSeq Adapter, Index 8 (100% over 43bp)
ACACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC396390.10184548991665673TruSeq Adapter, Index 8 (100% over 44bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT393300.10105156836504728TruSeq Adapter, Index 8 (100% over 48bp)
AACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC391340.10054798058473839TruSeq Adapter, Index 8 (100% over 44bp)
ACCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT390380.10030132534540341TruSeq Adapter, Index 8 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)