FastQCFastQC Report
Wed 16 May 2018
EPI-44_S41_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-44_S41_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38920722
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC9145092.3496712111352918Illumina Single End PCR Primer 1 (100% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC2184150.5611792093682126Illumina Single End PCR Primer 1 (100% over 49bp)
GGCTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1653310.4247891393176108Illumina Single End PCR Primer 1 (100% over 44bp)
GGCCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1364150.3504945257695887Illumina Single End PCR Primer 1 (100% over 44bp)
GGCGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1231650.31645096409054285Illumina Single End PCR Primer 1 (100% over 44bp)
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG1228150.3155517001971341Illumina Single End PCR Primer 1 (100% over 48bp)
GGGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1179900.30315470509514186Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1132270.2909170081685535Illumina Single End PCR Primer 1 (100% over 44bp)
GGGCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1125490.28917500554075026Illumina Single End PCR Primer 1 (100% over 44bp)
GGCGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1087150.2793242119198097Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG1073620.27584791464043246Illumina Single End PCR Primer 1 (100% over 45bp)
GGGGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1020480.2621945194130777Illumina Single End PCR Primer 1 (100% over 44bp)
GGCCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1017790.2615033708778578Illumina Single End PCR Primer 1 (100% over 44bp)
GGCGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG983580.2527137086511396Illumina Single End PCR Primer 1 (100% over 45bp)
GGCCGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG937320.24082800930568551Illumina Single End PCR Primer 1 (100% over 44bp)
GGGTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG914240.2348980062600072Illumina Single End PCR Primer 1 (100% over 44bp)
GCGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT913930.2348183571723053Illumina Single End PCR Primer 1 (100% over 46bp)
TGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG802570.20620634940944826Illumina Single End PCR Primer 1 (100% over 48bp)
GGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT802530.2061960721078093Illumina Single End PCR Primer 1 (100% over 46bp)
GTGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG775020.19912785790561646Illumina Single End PCR Primer 1 (100% over 44bp)
GGCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG743470.1910216362378889Illumina Single End PCR Primer 1 (100% over 45bp)
GGGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG736770.18930018821336358Illumina Single End PCR Primer 1 (100% over 45bp)
GGCAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG730750.1877534543167005Illumina Single End PCR Primer 1 (100% over 44bp)
GGCTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG730010.18756332423637978Illumina Single End PCR Primer 1 (100% over 44bp)
GGGCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG714410.18355517659718645Illumina Single End PCR Primer 1 (100% over 44bp)
GCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG713760.1833881704455534Illumina Single End PCR Primer 1 (100% over 48bp)
GGGGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG711970.18292826119721006Illumina Single End PCR Primer 1 (100% over 44bp)
GCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT701670.18028185602517857Illumina Single End PCR Primer 1 (100% over 46bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC694380.1784088178014786Illumina Single End PCR Primer 1 (100% over 49bp)
GCTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG662840.1703051654591608Illumina Single End PCR Primer 1 (100% over 45bp)
GGGCCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG639540.1643186372544682Illumina Single End PCR Primer 1 (100% over 44bp)
GGCCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG639220.16423641884135654Illumina Single End PCR Primer 1 (100% over 45bp)
GGTGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG628760.1615489044627692Illumina Single End PCR Primer 1 (100% over 44bp)
GGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG622800.1600175865185646Illumina Single End PCR Primer 1 (100% over 44bp)
GCGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG592390.15220426794754732Illumina Single End PCR Primer 1 (100% over 44bp)
GGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT591070.15186511699346172Illumina Single End PCR Primer 1 (100% over 46bp)
GGCGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG579060.1487793571763648Illumina Single End PCR Primer 1 (100% over 44bp)
GCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC574780.1476796859009964Illumina Single End PCR Primer 1 (100% over 47bp)
GTGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG574740.14766940859935745Illumina Single End PCR Primer 1 (100% over 45bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC558150.14340689774459991Illumina Single End PCR Primer 1 (100% over 49bp)
GCCTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG557200.14316281183067467Illumina Single End PCR Primer 1 (100% over 44bp)
GTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT556760.14304976151264615Illumina Single End PCR Primer 1 (100% over 46bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG553250.14214792829382764Illumina Single End PCR Primer 1 (100% over 48bp)
GGCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT544100.13979699554391617Illumina Single End PCR Primer 1 (100% over 46bp)
GCCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG531100.13645687251125505Illumina Single End PCR Primer 1 (100% over 45bp)
GGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG491360.12624637333295102Illumina Single End PCR Primer 1 (100% over 45bp)
GGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT490930.12613589234033223Illumina Single End PCR Primer 1 (100% over 46bp)
GTGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG487000.12512614745430467Illumina Single End PCR Primer 1 (100% over 44bp)
GGCCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG483500.12422688356089591Illumina Single End PCR Primer 1 (100% over 45bp)
GTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT482430.1239519657420538Illumina Single End PCR Primer 1 (100% over 46bp)
TGGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG482170.12388516328140059Illumina Single End PCR Primer 1 (100% over 44bp)
TGGGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG476420.12240780117080048Illumina Single End PCR Primer 1 (100% over 44bp)
GGCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG470030.12076600223397706Illumina Single End PCR Primer 1 (100% over 44bp)
GCGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG462090.11872595785864404Illumina Single End PCR Primer 1 (100% over 45bp)
GGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG460120.11821980075292539Illumina Single End PCR Primer 1 (100% over 45bp)
GTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC440540.11318906160065582Illumina Single End PCR Primer 1 (100% over 47bp)
GGCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG438700.11271630572526378Illumina Single End PCR Primer 1 (100% over 44bp)
GTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG436460.11214077683348218Illumina Single End PCR Primer 1 (100% over 48bp)
GCGTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG434340.11159607984661743Illumina Single End PCR Primer 1 (100% over 44bp)
GCGGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG429370.11031912511797701Illumina Single End PCR Primer 1 (100% over 44bp)
GGCGTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT425590.10934792011309555Illumina Single End PCR Primer 1 (100% over 43bp)
GGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT420560.10805554943199666Illumina Single End PCR Primer 1 (100% over 46bp)
GCCGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG419950.10789882058200256Illumina Single End PCR Primer 1 (100% over 44bp)
GGGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG413990.10636750263779793Illumina Single End PCR Primer 1 (100% over 45bp)
GGCAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG412710.1060386289853513Illumina Single End PCR Primer 1 (100% over 44bp)
GGGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG409660.10525498473538081Illumina Single End PCR Primer 1 (100% over 45bp)
GCTGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG408330.10491326445588547Illumina Single End PCR Primer 1 (100% over 44bp)
GGGCCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG408300.10490555647965626Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG405100.10408337234853968Illumina Single End PCR Primer 1 (100% over 44bp)
TGGGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG401500.10315841520103353Illumina Single End PCR Primer 1 (100% over 44bp)
GGCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG394950.10147550705765426Illumina Single End PCR Primer 1 (100% over 45bp)
GCGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG394180.10127766900110435Illumina Single End PCR Primer 1 (100% over 44bp)
GGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC392450.10083317570521945Illumina Single End PCR Primer 1 (100% over 47bp)
GCCAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG391700.100640476299489Illumina Single End PCR Primer 1 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)