Bismark report for: /home/sam/analyses/20180503_oly_methylseq_trimgalore/zr1394_8_s456_trimmed.fq.gz (version: v0.19.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /home/sam/data/oly_methylseq/20180503_oly_genome_pbjelly_sjw_01_bismark/ with the specified options: -q --score-min L,0,-0.2 -p 16 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 38697189 Number of alignments with a unique best hit from the different alignments: 12575055 Mapping efficiency: 32.5% Sequences with no alignments under any condition: 10453397 Sequences did not map uniquely: 15668737 Sequences which were discarded because genomic sequence could not be extracted: 25167 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 2775397 ((converted) top strand) CT/GA: 2761832 ((converted) bottom strand) GA/CT: 3516452 (complementary to (converted) top strand) GA/GA: 3496207 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 76972639 Total methylated C's in CpG context: 11089716 Total methylated C's in CHG context: 334631 Total methylated C's in CHH context: 3887465 Total methylated C's in Unknown context: 2216 Total unmethylated C's in CpG context: 1652203 Total unmethylated C's in CHG context: 15523342 Total unmethylated C's in CHH context: 44485282 Total unmethylated C's in Unknown context: 14395 C methylated in CpG context: 87.0% C methylated in CHG context: 2.1% C methylated in CHH context: 8.0% C methylated in Unknown context (CN or CHN): 13.3% Bismark completed in 12d 18h 13m 11s