FastQCFastQC Report
Tue 11 Sep 2018
1_ATCACG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1_ATCACG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9402890
Sequences flagged as poor quality0
Sequence length51
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAC252.8873257E-547.334971
CTCGTGC358.1492544E-847.3349651
CCTACGT301.5296719E-647.3349651
CCGTAGC205.483433E-447.3349651
AAGCCAA205.483433E-447.3349651
CTTATGC205.483433E-447.3349651
CTGCAGT301.5296719E-647.3349651
CCAACGT205.483433E-447.3349651
CCCGGGC205.483433E-447.3349651
CTTAGTC205.483433E-447.3349651
CAATGCG650.043.693821
CATCTGG402.3276334E-741.4180951
CTTGTGC354.417878E-640.5728261
CTATGTC354.417878E-640.5728261
CTCAAGG354.417878E-640.5728261
CTAAGAC354.417878E-640.5728261
CTCATGG354.417878E-640.5728261
ACCGTGC354.417878E-640.5728261
CAGTAGC354.417878E-640.5728261
CTGGGAC354.417878E-640.5728261
Produced by FastQC (version 0.11.5)