FastQCFastQC Report
Tue 11 Sep 2018
2_CGATGT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2_CGATGT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11954873
Sequences flagged as poor quality0
Sequence length51
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGC551.6734703E-938.742721
CTAACGC250.001641354237.881771
CTACGAA455.8448313E-736.82951
CTTAGGC652.1282176E-1036.424781
CTTGTAC557.2170224E-834.4379731
TCCATGG557.2170224E-834.4379731
CCCGTAC352.0924266E-433.823011
CAAGCGG352.0924266E-433.823011
CCGTGAC501.3325935E-633.146551
ACCGTAC501.3325935E-633.146551
CCGTAGT2950.032.9057731
CCTGTAT6150.032.7230721
CTTGGGT15050.032.407161
CCTAGCG300.00401316531.5681441
CCATGCT300.00401316531.5681441
CTTATGC300.00401316531.5681441
CTACGGT1350.031.5681441
CTCGCGC300.00401316531.5681441
GGTGCCT300.00401316531.5681441
CCGCAGT300.00401316531.5681441
Produced by FastQC (version 0.11.5)