FastQCFastQC Report
Tue 11 Sep 2018
4_TGACCA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4_TGACCA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11606618
Sequences flagged as poor quality0
Sequence length51
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAAG301.5335736E-647.3176231
CTATGAC404.3764885E-947.317621
ACACGAA252.8936329E-547.317621
TCACAGG205.4934074E-447.317621
CTGGTCC205.4934074E-447.317621
ACTAGGC252.8936329E-547.317621
CTGTGGC358.173083E-847.3176161
TGACAGC252.9023748E-547.2934722
TCCCGGT553.6379788E-1143.0160181
CTCAGTG553.6379788E-1143.0160181
CTCATGT506.6211214E-1042.5858571
CTCAGGA506.6211214E-1042.5858571
CTGGTAC1600.041.4029161
CCGTAGC402.3344182E-741.4029161
CTTGGCT354.4291155E-640.5579571
CCAAGAA354.4291155E-640.5579571
CTTAGTC354.4291155E-640.5579571
CTCGTAC308.485558E-539.431351
CCTAGCG308.485558E-539.431351
CTGAGGC308.485558E-539.431351
Produced by FastQC (version 0.11.5)