FastQCFastQC Report
Tue 11 Sep 2018
5_ACAGTG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5_ACAGTG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12589609
Sequences flagged as poor quality0
Sequence length51
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAGTA205.490437E-447.3228451
CCTAGGC252.8917602E-547.322841
CCTAGAC252.8917602E-547.322841
CTTGCGC358.165989E-847.322841
CCGAGAC358.165989E-847.322841
CTCTAGG1050.042.8159031
ACCGGAC402.3324174E-741.407491
CCCGCGA402.3324174E-741.407491
CCGGGAC354.4257904E-640.5624351
TACCAGG354.4257904E-640.5624351
CTAAGTC354.4257904E-640.5624351
CTTAGAC700.040.5624351
CCGTAGC609.094947E-1139.43571
CAGTAGC308.480088E-539.43571
CTTCGCG850.038.9717521
CCGAGAA850.038.9717521
CTCATGT850.038.9717521
CTACTGG250.00164636837.8582731
ACTGAAC250.00164636837.8582731
AATGCAC250.00164636837.8582731
Produced by FastQC (version 0.11.5)