FastQCFastQC Report
Tue 11 Sep 2018
6_GCCAAT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename6_GCCAAT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12489766
Sequences flagged as poor quality0
Sequence length51
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCGG252.8910712E-547.324751
CTTAGAC358.1634425E-847.3247451
CTTAGGC609.094947E-1139.437291
CTTACGC308.4780695E-539.437291
CTGCGAT750.037.8598021
AGGCCCG250.00164604237.85981
CCGGTAC250.00164604237.85981
TCAGCTT250.001649264637.8447272
TATCCTG250.001651255537.8354343
CCATGTA2350.037.2556531
CCAATGT900.036.8081361
CTTAACG401.10611945E-535.493561
CTCGTAC401.10611945E-535.493561
CCGAGAC603.939931E-935.493561
CCAAGGT1800.035.493561
TTCCGTG557.253948E-834.4181
CTAGTAC557.253948E-834.4181
CACTGGT1600.034.0146641
CCTAGGT4750.033.8745571
CTCACGT352.0995758E-433.803391
Produced by FastQC (version 0.11.5)