FastQCFastQC Report
Tue 11 Sep 2018
8_ACTTGA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8_ACTTGA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14374642
Sequences flagged as poor quality0
Sequence length51
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTAAC252.8816561E-547.350941
CCTAGAC205.4744224E-447.350941
CCAAGTG451.2320015E-842.0897251
CATATGC402.3215944E-741.432071
GCTCAGG354.4078206E-640.586521
CTATGCA308.450545E-539.4591181
CTTGGCA308.450545E-539.4591181
CTTATGC308.450545E-539.4591181
CCTATGC308.450545E-539.4591181
CTCGTGA250.001641582837.8807531
CTGAGGC250.001641582837.8807531
AAGCCCA250.001641582837.8807531
CAGGCAT250.001641582837.8807531
TCCGTAC250.001641582837.8807531
CTTAGGC1000.037.8807531
CTCAGGA1000.037.8807531
CGTCTCA301.1807218E-437.28024344
CAATGGC701.0913936E-1137.204311
CAACGTG652.1282176E-1036.42381
CTATGGC1200.035.5132061
Produced by FastQC (version 0.11.5)