FastQCFastQC Report
Tue 11 Sep 2018
3_TTAGGC_L001_R1_001_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename3_TTAGGC_L001_R1_001_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11758842
Sequences flagged as poor quality0
Sequence length20-43
%GC24

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCTAC250.003022784433.44865836
GACTTAC604.7293724E-1133.042939
CTAGCAG451.3301542E-732.12236817
TGTGCTC4100.031.6449551
GACGCTC1800.030.0334071
TCTAGCA503.3750803E-728.88463816
TTGCTCT250.006246243628.8374658
ACGTCTG450.00851714927.06221437
GCCGATG1200.027.0300671
TTCTAGC557.8236917E-726.23529415
CCAACTC359.426755E-426.15655325
TTACTCA708.720235E-925.74828512
CGCTCTT2050.024.6127413
ACGCTCT2400.021.7742212
CCGATGA1500.021.6240522
GTGCTCT6200.021.217152
CTTACTC1051.364242E-920.59836611
GCTCTTC8200.019.558344
ACTTACT1203.4015102E-1019.52536810
GCCTCTC1900.019.04361318
Produced by FastQC (version 0.11.5)