FastQCFastQC Report
Mon 10 Sep 2018
2112_lane1_GCCAAT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_GCCAAT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24792684
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC249108510.047661640829206TruSeq Adapter, Index 6 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGT1672440.6745699658818706TruSeq Adapter, Index 6 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1512300.6099783307043319TruSeq Adapter, Index 6 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC813800.3282419926781626TruSeq Adapter, Index 6 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC595870.24034106190358412TruSeq Adapter, Index 6 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC391700.15799015548296425TruSeq Adapter, Index 6 (98% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCT375260.15135916708332184TruSeq Adapter, Index 6 (100% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC341980.13793585236677078TruSeq Adapter, Index 6 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)