FastQCFastQC Report
Mon 10 Sep 2018
2112_lane1_TTAGGC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_TTAGGC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25752634
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC280275710.88337992921423TruSeq Adapter, Index 3 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1662960.6457436548043979TruSeq Adapter, Index 3 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGT1606490.623815800744887TruSeq Adapter, Index 3 (100% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC917720.3563596640250469TruSeq Adapter, Index 3 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC654950.254323499491353TruSeq Adapter, Index 3 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC403290.15660145676749027TruSeq Adapter, Index 3 (98% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC380550.14777129205501852TruSeq Adapter, Index 3 (98% over 50bp)
GATCCGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC337220.13094582868688306TruSeq Adapter, Index 3 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)