Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/data/O_lurida/BSseq/whole_genome_BSseq_reads/20180830_trimgalore/6_GCCAAT_L001_R1_001_trimmed.fq.gz

Data processed at 01:47 on 2018-09-13


Alignment

Sequences analysed in total 12409649
Single-end alignments with a unique best hit 5269110
Sequences without alignments under any condition 3275362
Sequences that did not map uniquely 3865177
Genomic sequence context not extractable (edges of chromosomes) 10014

Cytosine Methylation

Total C's analysed 40940100
Methylated C's in CpG context 1640749
Methylated C's in CHG context 127541
Methylated C's in CHH context 942648
Methylated C's in Unknown context 1916
Unmethylated C's in CpG context 4498550
Unmethylated C's in CHG context 8020739
Unmethylated C's in CHH context 25709873
Unmethylated C's in Unknown context 34838
Percentage methylation (CpG context) 26.7%
Percentage methylation (CHG context) 1.6%
Percentage methylation (CHH context) 3.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2577697 original top strand
CTOT 55210 complementary to original top strand
CTOB 50573 complementary to original bottom strand
OB 2575616 original bottom strand