Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/data/O_lurida/BSseq/whole_genome_BSseq_reads/20180830_trimgalore/5_ACAGTG_L001_R1_001_trimmed.fq.gz

Data processed at 22:30 on 2018-09-13


Alignment

Sequences analysed in total 12502181
Single-end alignments with a unique best hit 6565485
Sequences without alignments under any condition 1566378
Sequences that did not map uniquely 4370318
Genomic sequence context not extractable (edges of chromosomes) 16973

Cytosine Methylation

Total C's analysed 50755672
Methylated C's in CpG context 1984755
Methylated C's in CHG context 149001
Methylated C's in CHH context 1826039
Methylated C's in Unknown context 28729
Unmethylated C's in CpG context 5498703
Unmethylated C's in CHG context 9800659
Unmethylated C's in CHH context 31496515
Unmethylated C's in Unknown context 160224
Percentage methylation (CpG context) 26.5%
Percentage methylation (CHG context) 1.5%
Percentage methylation (CHH context) 5.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3136437 original top strand
CTOT 131919 complementary to original top strand
CTOB 135994 complementary to original bottom strand
OB 3144162 original bottom strand