FastQCFastQC Report
Fri 20 Mar 2020
113_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename113_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31638462
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA31597979.987201653481133No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT20663366.531088647735152No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG1825370.5769465026460515No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCA1273480.402510084086894No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGC1102000.3483102307564761No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTC929520.29379430643626103No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTTAT907600.2868660303399072No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTC903610.2856049070906165No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTT901320.28488110452398097No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTG876140.27692243700088837No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTTATAC839220.265253096057577No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTTATACAC684920.21648334233187444No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA569670.1800561607577511No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTTATACACAT562580.1778152174400892No Hit
GTATCAACGCAGAGTACGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA542450.17145270841547228No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTTATACACATCT498460.157548745574295No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT497030.15709676405888504No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCACT495560.1566321397038832No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTGTCTCTTATACACATCTCC352200.1113202026065616No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCACTCT346710.10958497287257515No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCC332550.10510940765704731No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)