FastQCFastQC Report
Fri 20 Mar 2020
222_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename222_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53808137
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA771940514.34616664018678No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT718335213.349936274508073No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA6272871.1657846470321023No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT6085371.1309386162170973No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACC1889990.3512461321602716No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGA1347820.25048627868309215No Hit
CACACACACACACACACACACACACACACACACACACACCTGTCTCTTAT1297500.24113453323983325No Hit
CACACACACACACACACACACACACACACACACACACACACACCTGTCTC1288740.23950652668015618No Hit
CACACACACACACACACACACACACACACACACACACACACCTGTCTCTT1281690.23819631592151203No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG1272760.2365367156272294No Hit
CACACACACACACACACACACACACACACACACACACACACACACCTGTC1260350.2342303730010203No Hit
CACACACACACACACACACACACACACACACACACACCTGTCTCTTATAC1246890.2317288926022471No Hit
CACACACACACACACACACACACACACACACACACACACACACACACCTG1223110.2273094866674161No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGC1149840.21369258705240066No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTAT1102670.20492625492683383No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATAC1073830.1995664707737419No Hit
GTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1072810.19937690836610827No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTT1069500.19876175976878738No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTC1065420.1980035101382529No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTC1046090.194411116668098No Hit
CACACACACACACACACACACACACACACACACACCTGTCTCTTATACAC1041660.19358782111337547No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTG1007200.1871835852633218No Hit
CACTCACACACACACACACACACACACACACACACACACACACACACACA981130.18233859313880355No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACAC922860.17150937598898844No Hit
CACACACACACACACACACACACACACACACACCTGTCTCTTATACACAT874820.16258135828044No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACAT785570.1459946476124977No Hit
CACACACACACACACACACACACACACACACCTGTCTCTTATACACATCT779350.14483868861692795No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGT747810.13897712162009995No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCT723990.13455028186536175No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTT699930.13007883919118032No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC640680.1190674934536388No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGTGT623000.11578174505465595No Hit
CACATACACACACACACACACACACACACACACACACACACACACACACA622470.11568324694088553No Hit
GTGTGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT620540.11532456513036309No Hit
CTCACACACACACACACACACACACACACACACACACACACACACACACA618850.11501048623928384No Hit
CACCCACACACACACACACACACACACACACACACACACACACACACACA599660.11144411113880415No Hit
GTGTCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT589750.10960238225679511No Hit
CACACACACACACACACACACACACACACCTGTCTCTTATACACATCTGA562800.10459384609431842No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCTGA540800.10050524514535784No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)