FastQCFastQC Report
Fri 20 Mar 2020
334_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename334_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25916895
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA1046040.40361316430845595No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA777140.2998584514078558No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGGCGG629140.24275284520001333No Hit
GTACTAAGATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAG588530.22708352987501013No Hit
GATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAGTCATGAG567030.21878778302724922No Hit
TCTCTAGAGAGCACGGAGTCCTGTGCTTCGTGCATGAAGGCTATGTGCTT524140.2022387326876927No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCGAGCCTTTCATTGGCGG521560.20124324306596142No Hit
GTATCAACGCAGAGTACGGGACACACGACGCTTGGCATCCTCGACAACAT499740.1928240246372106No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT483770.18666202104843194No Hit
TTTATATACTCTACAAGTTTTTTTCTGCTTTTCATTATAGGGACAAGGCC472720.18239839301737343No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG461210.17795727458864188No Hit
TTATTAGACAACACAAAGAACTGCTGGATCGGAACTCTTGCATTCTGGAT430850.16624290834222233No Hit
CAATTATTTATTGCAAATATTTTCACGTGTATGGAAAGTAAATTAACACT399030.1539652030075362No Hit
GTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAAGTTGGACAGCACCTGC398410.1537259768193682No Hit
AACAAGGACCTGCCATGCTACACGGACAGAATTCTGAGCAATGCCGAGAA377010.14546881484066668No Hit
CTTTAGAACTAACAACACAAACTCAAGAGAAAATAAGTGCTTCACAATTT368280.14210035577178515No Hit
TTGTTAGCCCTTGTAAGTTGGACAGCACCTGCCCCAGCGCCTGCAGCAGA360730.139187198157804No Hit
GTATATAAATGAATTGTCTACTGTGACATGGGGTTACAGATAGATTTCTT358550.13834604801231012No Hit
GCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAA348130.13432550465632553No Hit
GGCTAACAAGGACCTGCCATGCTACACGGACAGAATTCTGAGCAATGCCG309080.1192581132886482No Hit
ATTATATACTAACTTTCACTGCTTTCAAACGTTTTTATTGAGTCATGAGT297190.11467037235749114No Hit
GTCCAAGTACCTACGGCGGGCGTGGCATCTGCTGCAGGCGCTGGGGCAGG294390.11358999602382924No Hit
TATCAACGCAGAGTACGGGACACACGACGCTTGGCATCCTCGACAACATC291220.11236685567464774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289640.11175721474350998No Hit
GTCTAATAATATTCAGCAGTCTTGCATACGTGGTGCTTCAGGCAGACGTC277960.10725050203737754No Hit
CAATTAAGGAAGTCAATAGTCACCTGAATAGCAAACCAATGATGCAAGAT274550.10593475800245362No Hit
ATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGC269860.10412512764356996No Hit
ATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGT269840.10411741066975809No Hit
CTCTAAGCAATTAAGGAAGTCAATAGTCACCTGAATAGCAAACCAATGAT268740.1036929771101052No Hit
ACTTACAAGGGCTAACAAGGACCTGCCATGCTACACGGACAGAATTCTGA265790.1025547234728543No Hit
ATCCAGAACTAGCTGCTCCAGCCAGTGTCCAAGTACCTACGGCGGGCGTG265720.10252771406451275No Hit
GCTCCAGCCAGTGTCCAAGTACCTACGGCGGGCGTGGCATCTGCTGCAGG264920.10221903511203792No Hit
ATGCTACACGGACAGAATTCTGAGCAATGCCGAGAATGTACTAAGATTAT263720.10175601668332569No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)