FastQCFastQC Report
Tue 14 Apr 2020
380820_S1_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename380820_S1_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9346447
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAACTCCGATCTCGT670290.7171602214188986TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAACTCCGATCGCGT178430.19090676917121555TruSeq Adapter, Index 9 (97% over 36bp)
CTCGGAACCACCGGTGAATCCAGGCAGGGTCTGGACTCCATGAAATCCAG132240.14148692010985564No Hit
GCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAAAG106100.11351907307664613No Hit
CACCGGTGAATCCAGGCAGGGTCTGGACTCCTTGAAATCCAGGCTTGGTA104030.11130432773009895No Hit
CTGGGAACCACCGGTGAATCCAGGCAGGGTCTGGACTCCTTGAAATCCAG102910.11010601140732942No Hit
CACCGGTGAATCCAGGCAGGGTCTGGACTCCATGAAATCCAGGCCTGTTC102700.10988132709681016No Hit
CACCAGTACTTGCAGTCTCGCGGTTGCGGCCATAAGCTTGCCTCTGTCAC100650.10768798025602669No Hit
GTGCTTCACAATTTACTCATGACTCAATAAAAACGTTTGAAAGCAGTGAA98070.1049275730125041No Hit
CTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCAGGTCCTTGTTAG95760.10245604559679201No Hit
CTCGCGGTTGCGGCCATAAGCTTGCCTCTGTCACTGCCACCATTGCAACC95120.10177129341235232No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)