FastQCFastQC Report
Tue 14 Apr 2020
380822_S3_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename380822_S3_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9453032
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTC450870.4769580807512341No Hit
CGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGG284590.30105684609974875No Hit
CTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCT237370.2511046191317241No Hit
CTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAATGAAC213380.22572651822187845No Hit
CACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAAT213090.22541973834426882No Hit
CGCTTGGCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGC178580.18891293290872177No Hit
CATGGAGCGACGCACCCTGCTGGTTGTCCTGCTGGTGTGCAGCCTGGTGC178420.188743675045213No Hit
CAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGC169880.17970953658043262No Hit
ATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGC148770.15737807721374475No Hit
GCTACACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAA137320.14526556135639868No Hit
GAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAATGAACATGAAGG136660.14456737266942501No Hit
CTCTCCTGCCTTGACTACACTGCCTACTCGAAAAATGAACATGAAGGTGT112490.118998856663132No Hit
GTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAATGAACAT110320.11670329688929437No Hit
GCATCCTCGACAACATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCG108640.1149260893224523No Hit
CGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAATGAACATGAAG108470.11474625284247425No Hit
CATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACGCCG107400.11361434088025936No Hit
ACACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCGAAAAA102830.10877991315379022No Hit
CAAGGACCTGCCATGCTACACGGACAGAATTCTGAGCAATGCCGAGAATG97520.10316266780859307No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)