FastQCFastQC Report
Fri 20 Mar 2020
425_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename425_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66224932
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA1306983019.735512903206907No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1032021415.583578100163242No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA22513063.399484049300345No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT15292422.3091635639580574No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACC3348550.5056328332658764No Hit
CACACACACACACACACACACACACACACACACACACACACACCTGTCTC2331780.3521000217863568No Hit
CACACACACACACACACACACACACACACACACACACACACACACCTGTC2297820.34697204370100376No Hit
CACACACACACACACACACACACACACACACACACACACACCTGTCTCTT2278020.34398223315653986No Hit
CACACACACACACACACACACACACACACACACACACACACACACACCTG2273560.3433087707813728No Hit
CACACACACACACACACACACACACACACACACACACACCTGTCTCTTAT2268980.3426171883422998No Hit
CACACACACACACACACACACACACACACACACACACCTGTCTCTTATAC2169800.3276409555241219No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGC2034130.3071547132732428No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG2033780.3071018630868507No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGA2005420.30281948798377023No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTAT1905740.287767754899318No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTC1892390.28575189778979315No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTT1864050.2814725426973636No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTC1862930.2813034221009091No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATAC1826050.27573452246051383No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTG1817070.2743785376782267No Hit
CACTCACACACACACACACACACACACACACACACACACACACACACACA1812780.2737307453935928No Hit
CACACACACACACACACACACACACACACACACACCTGTCTCTTATACAC1781620.26902556879937606No Hit
GTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1652130.2494725098396477No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACAC1477410.22308969679274265No Hit
CACACACACACACACACACACACACACACACACCTGTCTCTTATACACAT1448760.21876353153522302No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG1393810.21046605227167314No Hit
CACACACACACACACACACACACACACACACCTGTCTCTTATACACATCT1267110.1913342847977556No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACAT1237900.18692355924200874No Hit
CACATACACACACACACACACACACACACACACACACACACACACACACA1197520.1808261577376931No Hit
CACCCACACACACACACACACACACACACACACACACACACACACACACA1160560.17524517805469397No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCT1094670.16529575296506155No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGT1085510.16391258808691567No Hit
GTGTGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1079850.16305792507269015No Hit
GTGTCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT997240.15058377107884385No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC950550.14353355621414607No Hit
CACACACACACACACACACACACACACACACACACACACACACACACAAA926770.1399427635501385No Hit
CTCACACACACACACACACACACACACACACACACACACACACACACACA902950.13634593086482896No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGTGT879040.13273550813159007No Hit
CACACACACACACACACACACACACACACCTGTCTCTTATACACATCTCC870420.13143388354102048No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCA833490.12585743387399778No Hit
GTGTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT828530.12510847123255636No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCTCC756800.11427720303283966No Hit
CACAAACACACACACACACACACACACACACACACACACACACACACACA730350.11028323894692713No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGC725440.10954182633211311No Hit
CACACACACACACACACACACACACACACACACACACACACACACAAACA724800.10944518599128195No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGTGTGT717870.10839875230071962No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTT703780.10627115479710873No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)