FastQCFastQC Report
Fri 20 Mar 2020
427_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename427_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18918640
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT4906972.593722381735685No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGT3160281.6704583416144079No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA2665361.4088539133891231No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA2281271.2058319202648815No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT2127191.1243884338409105TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT1873140.9901028826596415RNA PCR Primer, Index 12 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCT1594790.8429728563998257Illumina PCR Primer Index 12 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTT1486290.7856220108845032No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCT1222370.6461193827886148TruSeq Adapter, Index 12 (96% over 27bp)
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1217420.6435029156429849No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCTGCT993330.5250535979330438Illumina PCR Primer Index 12 (96% over 25bp)
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT655630.34655239488673606No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTG588260.3109420127451022TruSeq Adapter, Index 12 (96% over 25bp)
CACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCTG564410.298335398316158Illumina PCR Primer Index 12 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC476070.25164070990303744No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTC322110.17026065298562687No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG303470.1604079362998609TruSeq Adapter, Index 12 (96% over 29bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGCGTAGT285210.15075607971820384No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT274960.1453381426994752RNA PCR Primer, Index 12 (95% over 21bp)
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGC258280.1365214412875344Illumina PCR Primer Index 5 (96% over 25bp)
TCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCTGCTTG244240.12910018901992956Illumina PCR Primer Index 12 (96% over 27bp)
ACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCTGC226940.11995576849075833Illumina PCR Primer Index 12 (95% over 24bp)
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG199720.10556784208590048No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTTTGCCGT190680.1007894859249925No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)