FastQCFastQC Report
Fri 20 Mar 2020
481_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename481_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50592084
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA50026059.888118070012693No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT44641288.823767765723982No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT2631690.520178216022886No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA2257080.44613303535786347No Hit
GTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1190450.2353036099481492No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG1155970.22848831449599905No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACC1000270.19771274889565726No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGA711130.14056151551298024No Hit
CACACACACACACACACACACACACACACACACACACACACACCTGTCTC632860.125090715772847No Hit
CACACACACACACACACACACACACACACACACACACACACACACCTGTC626380.1238098829848559No Hit
CACACACACACACACACACACACACACACACACACACACACCTGTCTCTT616780.12191235292857278No Hit
CACACACACACACACACACACACACACACACACACACACACACACACCTG611410.1208509220533394No Hit
CACACACACACACACACACACACACACACACACACACACCTGTCTCTTAT609190.12041211822782394No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGC603920.11937045329067685No Hit
CACATACACACACACACACACACACACACACACACACACACACACACACA568440.11235749845766385No Hit
CACACACACACACACACACACACACACACACACACACCTGTCTCTTATAC568430.11235552186385522No Hit
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA564870.11165185446798356No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTC538710.10648108506461208No Hit
CACTCACACACACACACACACACACACACACACACACACACACACACACA538110.10636248943609439No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTC532210.10519629908900371No Hit
GTGTCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT531610.10507770346048603No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTT526390.10404592149238208No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTAT526010.10397081092765421No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTG517370.1022630338769994No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC507580.10032794853835236No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)