FastQCFastQC Report
Fri 20 Mar 2020
485_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename485_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26010208
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCCAGCCTTTCATTGGCGG663210.25498065990091273No Hit
ATCACCATATTGGGGACGTTGGAAGGGTCGTGTGGAGCCAATGCCGCCGC644610.24782962135481576No Hit
ATCACCATATTGGGGACGTTGGATGGGTCGTGTGGAGCCAATGCCGCCGC572450.2200866675114632No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT526890.2025704677179052No Hit
TCTCTAGAGAGCACGGAGTCCTGTGCTTCGTGCATGAAGGCTATGTGCTT478590.1840008353643308No Hit
GTGTAGCATGGCAGGTCCTTGTTAGCCCTTGTAAGTTGGACAGCACCTGC442240.1700255530444047No Hit
TCACCATATTGGGGACGTTGGAAGGGTCGTGTGGAGCCAATGCCGCCGCC441660.16980256367038665No Hit
GTATATAATCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTA433780.1667729838992445No Hit
GTCCAGACCCTGCCTGGATTCACCGGTGGTTCCGAGCCTTTCATTGGCGG408790.15716521759456903No Hit
GTATCAACGCAGAGTACGGGACACACGACGCTTGGCATCCTCGACAACAT398560.1532321463942157No Hit
TCTTAGTACATTCTCGGCATTGCTCAGAATTCTGTCCGTGTAGCATGGCA388510.14936827879269554No Hit
AGTTTTCATCAAGAACAGGCCTGGATTTCAAGGAGTCCAGACCCTGCCTG387010.14879158213575225No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA385820.14833406945457722No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371870.1429707905450045No Hit
AGTTTTCATCAAGAACAGGCCTGGATTTCATGGAGTCCAGACCCTGCCTG368940.14184430974177523No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT359040.1380381118059494No Hit
TTGTTAGCCCTTGTAAGTTGGACAGCACCTGCCCCAGCGCCTGCAGCAGA330690.12713854498972096No Hit
CATCATGCGTGTCCTGTTAATCCTGGTCAGCCTGGCGGCTCTGGTGCACG313840.12066031921005783No Hit
TCACCATATTGGGGACGTTGGATGGGTCGTGTGGAGCCAATGCCGCCGCC274380.1054893524880693No Hit
GAGCTACACACTGTGGCGAACTCTCCTGCCTTGACTACACTGCCTACTCG262890.1010718560958836No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)