FastQCFastQC Report
Fri 20 Mar 2020
73_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename73_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25856008
Sequences flagged as poor quality0
Sequence length150
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT17516366.7745802058848374No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGT13235515.118930192162688No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT9595423.7110987898828003Illumina PCR Primer Index 10 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTT8683263.3583142455710875No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT6067532.3466615573448153Illumina PCR Primer Index 10 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTTCT5991062.3170862261490637RNA PCR Primer, Index 12 (95% over 21bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA2608631.0089067113531214No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT2595311.0037551040361683Illumina PCR Primer Index 10 (96% over 26bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTTCTGCT2451080.9479730977806009TruSeq Adapter, Index 12 (95% over 24bp)
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT2383720.921921125643216No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTTCTG1444140.5585316959988564RNA PCR Primer, Index 29 (95% over 23bp)
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG1298670.5022701106837528Illumina PCR Primer Index 10 (95% over 24bp)
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG1242230.48044152832873505Illumina PCR Primer Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTTCTGCTTG1223340.4731356828169298TruSeq Adapter, Index 12 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC1213570.46935706393655197No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1113820.43077802265531473No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTC1035620.4005336013200491No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCT958710.3707880969096235No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCT842470.3258314276511672No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT792100.30635046214404016No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACACTCGCT559500.21639071275039828No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGC524080.20269176896913088Illumina PCR Primer Index 10 (96% over 25bp)
ACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTTCTGC523630.20251772818139596RNA PCR Primer, Index 29 (95% over 24bp)
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA272410.1053565577485898Illumina PCR Primer Index 10 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)