nightingales/E5-coral-deep-dive-expression/genohub2216545


Whole genome bisulfite sequencing data.

Part of https://github.com/urol-e5/deep-dive-expression

| Number | Species                  | Timepoint | Collection Date | Site  | colony_id | Extraction Date | Extraction notebook post                                                                                                     | DNA (ng/uL) | Volume eluted | Total DNA (ng) | Primer | Date Prepped | Library concentration (ng/uL) | bp peak | Library volume (uL) | Prep notebook post                                                                                                            | Notes                                                                                                                                                           |
|--------|--------------------------|-----------|-----------------|-------|-----------|-----------------|------------------------------------------------------------------------------------------------------------------------------|-------------|---------------|----------------|--------|--------------|-------------------------------|---------|---------------------|-------------------------------------------------------------------------------------------------------------------------------|-----------------------------------------------------------------------------------------------------------------------------------------------------------------|
| 403    | Pocillopora tuahiniensis | TP2       | 20200305        | Site1 | POC-48    | 20211129        | https://github.com/Kterpis/Putnam_Lab_Notebook/blob/master/_posts/2021-11-29-20211129-RNA-DNA-extractions-from-E5-project.md | 29.1        | 90            | 2619           | 25     | 20240613     | 8.67                          | 253     | 14                  | https://github.com/JillAshey/JillAshey_Putnam_Lab_Notebook/blob/master/_posts/2024-06-13-Zymo-Pico-Methyl-Seq-Library-Prep.md | Labeled as POC-55 in KXT extraction post                                                                                                                        |
| 413    | Acropora pulchra         | TP2       | 20200305        | Site1 | ACR-178   | 20210902        | https://github.com/Kterpis/Putnam_Lab_Notebook/blob/master/_posts/2021-09-02-20210902-RNA-DNA-extractions-from-E5-project.md | 19.9        | 90            | 1791           | 26     | 20240613     | 9.64                          | 269     | 14                  | https://github.com/JillAshey/JillAshey_Putnam_Lab_Notebook/blob/master/_posts/2024-06-13-Zymo-Pico-Methyl-Seq-Library-Prep.md |                                                                                                                                                                 |
| 417    | Pocillopora tuahiniensis | TP2       | 20200305        | Site1 | POC-57    | 20211020        | https://kterpis.github.io/Putnam_Lab_Notebook/20211020-RNA-DNA-extractions-from-E5-project/                                  | 51.4        | 90            | 4626           | 27     | 20240613     | 9.3                           | 256     | 14                  | https://github.com/JillAshey/JillAshey_Putnam_Lab_Notebook/blob/master/_posts/2024-06-13-Zymo-Pico-Methyl-Seq-Library-Prep.md | Labeled as POC-238 in KXT extraction post                                                                                                                       |
| 423    | Acropora pulchra         | TP2       | 20200305        | Site1 | ACR-150   | 20210903        | https://github.com/Kterpis/Putnam_Lab_Notebook/blob/master/_posts/2021-09-03-20210903-RNA-DNA-extractions-from-E5-project.md | 17.7        | 90            | 1593           | 29     | 20240613     | 8.61                          | 267     | 14                  | https://github.com/JillAshey/JillAshey_Putnam_Lab_Notebook/blob/master/_posts/2024-06-13-Zymo-Pico-Methyl-Seq-Library-Prep.md | Labeled as POR-75 in KXT extraction post                                                                                                                        |
| 427    | Acropora pulchra         | TP2       | 20200305        | Site1 | ACR-145   | 20211012        | https://kterpis.github.io/Putnam_Lab_Notebook/20211012-RNA-DNA-extractions-from-E5-project/                                  | 19.6        | 90            | 1764           | 30     | 20240613     | 8.82                          | 271     | 14                  | https://github.com/JillAshey/JillAshey_Putnam_Lab_Notebook/blob/master/_posts/2024-06-13-Zymo-Pico-Methyl-Seq-Library-Prep.md |                                                                                                                                                                 |
| 439    | Acropora pulchra         | TP2       | 20200305        | Site1 | ACR-173   | 20211102        | https://kterpis.github.io/Putnam_Lab_Notebook/20211102-RNA-DNA-extractions-from-E5-project/                                  | 19.9        | 90            | 1791           | 31     | 20240613     | 11.9                          | 264     | 14                  | https://github.com/JillAshey/JillAshey_Putnam_Lab_Notebook/blob/master/_posts/2024-06-13-Zymo-Pico-Methyl-Seq-Library-Prep.md |                                                                                                                                                                 |
| 467    | Acropora pulchra         | TP2       | 20200305        | Site1 | ACR-140   | 20210921        | https://github.com/Kterpis/Putnam_Lab_Notebook/blob/master/_posts/2021-09-21-20210921-RNA-DNA-extractions-from-E5-project.md | 21.9        | 90            | 1971           | 32     | 20240613     | 16                            | 270     | 14                  | https://github.com/JillAshey/JillAshey_Putnam_Lab_Notebook/blob/master/_posts/2024-06-13-Zymo-Pico-Methyl-Seq-Library-Prep.md | DNA 1 and 2 Qubit readings are 27.6 and 27.4 but average is reported as 4.79 on github and on master sample sheet; JA re-qubited on 20240229 and got 21.9 ul/ng |
| 471    | Porites evermanni        | TP2       | 20200305        | Site1 | POR-76    | 20211015        | https://kterpis.github.io/Putnam_Lab_Notebook/20211015-RNA-DNA-extractions-from-E5-project/                                  | 2.7         | 90            | 243            | 33     | 20240613     | 5.25                          | 245     | 14                  | https://github.com/JillAshey/JillAshey_Putnam_Lab_Notebook/blob/master/_posts/2024-06-13-Zymo-Pico-Methyl-Seq-Library-Prep.md |                                                                                                                                                                 |
| 491    | Porites evermanni        | TP2       | 20200305        | Site1 | POR-73    | 20211101        | https://kterpis.github.io/Putnam_Lab_Notebook/20211101-RNA-DNA-extractions-from-E5-project/                                  | 2.11        | 90            | 189.9          | 36     | 20240613     | 4.65                          | 315     | 14                  | https://github.com/JillAshey/JillAshey_Putnam_Lab_Notebook/blob/master/_posts/2024-06-13-Zymo-Pico-Methyl-Seq-Library-Prep.md |                                                                                                                                                                 |
| 385    | Pocillopora tuahiniensis | TP2       | 20200305        | Site1 | POC-47    | 20211012        | https://kterpis.github.io/Putnam_Lab_Notebook/20211012-RNA-DNA-extractions-from-E5-project/                                  | 25.5        | 90            | 2295           | 22     | 20241015     | 4.95                          | 1477    | 14                  | https://github.com/JillAshey/JillAshey_Putnam_Lab_Notebook/blob/master/_posts/2024-10-15-Zymo-Pico-Methyl-Seq-Library-Prep.md |                                                                                                                                                                 |
| 393    | Pocillopora tuahiniensis | TP2       | 20200305        | Site1 | POC-50    | 20210903        | https://github.com/Kterpis/Putnam_Lab_Notebook/blob/master/_posts/2021-09-03-20210903-RNA-DNA-extractions-from-E5-project.md | 29.4        | 90            | 2646           | 23     | 20241015     | 0.05                          | 411     | 14                  | https://github.com/JillAshey/JillAshey_Putnam_Lab_Notebook/blob/master/_posts/2024-10-15-Zymo-Pico-Methyl-Seq-Library-Prep.md |                                                                                                                                                                 |
| 401    | Pocillopora tuahiniensis | TP2       | 20200305        | Site1 | POC-53    | 20211118        | https://kterpis.github.io/Putnam_Lab_Notebook/20211118-RNA-DNA-extractions-from-E5-project/                                  | 30.8        | 90            | 2772           | 24     | 20240807     | 3.82                          | 282     | 14                  | https://github.com/JillAshey/JillAshey_Putnam_Lab_Notebook/blob/master/_posts/2024-08-07-Zymo-Pico-Methyl-Seq-Library-Prep.md |                                                                                                                                                                 |
| 421    | Porites evermanni        | TP2       | 20200305        | Site1 | POR-82    | 20240925        | https://github.com/JillAshey/JillAshey_Putnam_Lab_Notebook/blob/master/_posts/2024-09-25-MiniprepPlus-DNA-extractions-E5.md  | 0.116       | 75            | 8.7            | 28     | 20241015     | 1.96                          | 485     | 14                  | https://github.com/JillAshey/JillAshey_Putnam_Lab_Notebook/blob/master/_posts/2024-10-15-Zymo-Pico-Methyl-Seq-Library-Prep.md |                                                                                                                                                                 |
| 487    | Porites evermanni        | TP2       | 20200305        | Site1 | POR-71    | 20211122        | https://github.com/Kterpis/Putnam_Lab_Notebook/blob/master/_posts/2021-11-22-20211122-RNA-DNA-extractions-from-E5-project.md | 0.954       | 80            | 76.32          | 34     | 20241015     | 4.26                          | 496     | 14                  | https://github.com/JillAshey/JillAshey_Putnam_Lab_Notebook/blob/master/_posts/2024-10-15-Zymo-Pico-Methyl-Seq-Library-Prep.md | Another large peak at 1616                                                                                                                                      |
| 489    | Porites evermanni        | TP2       | 20200305        | Site1 | POR-79    | 20211129        | https://github.com/Kterpis/Putnam_Lab_Notebook/blob/master/_posts/2021-11-29-20211129-RNA-DNA-extractions-from-E5-project.md | 0.804       | 80            | 64.32          | 35     | 20241015     | 3.87                          | 495     | 14                  | https://github.com/JillAshey/JillAshey_Putnam_Lab_Notebook/blob/master/_posts/2024-10-15-Zymo-Pico-Methyl-Seq-Library-Prep.md | Another large peak at 1447                                                                                                                                      |