FastQCFastQC Report
Tue 19 May 2026
lib_212398_sample_1703677_S99_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelib_212398_sample_1703677_S99_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9020057
Total Bases911 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGAGGAAATCTCGT386180.42813476677586404TruSeq Adapter, Index 3 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGAGGAAATCTCGG116200.12882401962648352TruSeq Adapter, Index 3 (97% over 38bp)
GCGAGACTTGCGTGATAAAAAGCTCAGAAGAAGCCCAAGAAGAAGAATAC108020.11975534079219233No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGAGGAAATCGCGT103480.11472211317511631TruSeq Adapter, Index 3 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)