FastQCFastQC Report
Tue 19 May 2026
lib_212413_sample_1703687_S104_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelib_212413_sample_1703687_S104_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8358319
Total Bases844.1 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAGTAGCAGATCTCGT592110.7084079944783156TruSeq Adapter, Index 5 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAGTAGCAGATCGCGT203180.24308715663998948TruSeq Adapter, Index 5 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAGTAGCAGATCTCGG148000.17706909726704617TruSeq Adapter, Index 5 (97% over 38bp)
GCGAGACTTGCGTGATAAAAAGCTCAGAAGAAGCCCAAGAAGAAGAATAC98700.11808594527200984No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAGTAGCAGATCGCGG87670.10488951187433741TruSeq Adapter, Index 5 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)