FastQCFastQC Report
Thu 6 Aug 2026
lib_213500_sample_1703763_S141_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelib_213500_sample_1703763_S141_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30349892
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTACTTCTAATCTCGT2494700.8219798607520581TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTACTTCTAATCTCGG1433210.47222902803080813TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTACTTCTAATCGCGT644330.21230059072368362TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTACTTCTAATCGCGG625650.20614570885458175TruSeq Adapter, Index 22 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)