FastQCFastQC Report
Thu 6 Aug 2026
lib_213523_sample_1703756_S135_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelib_213523_sample_1703756_S135_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25514251
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGACTAGCATCTCGG780910.3060681655910652TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGACTAGCATCTCGT274220.10747718990457529TruSeq Adapter, Index 7 (97% over 35bp)
GGAAAAATGGTTTGTTTAAATAAACTCTAGTGTACTTAGGGGGTATTAAT268270.10514515985595657No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGACTAGCATCGCGG262030.10269946783858165TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)